Self-assembled monolayers for biological applications: design, processing, characterization and biological studies

dc.contributor
Universitat Autònoma de Barcelona. Departament de Química
dc.contributor.author
R. Kyvik, Adriana
dc.date.accessioned
2019-05-14T06:12:05Z
dc.date.available
2021-01-24T01:00:28Z
dc.date.issued
2019-01-25
dc.identifier.isbn
9788449086007
en_US
dc.identifier.uri
http://hdl.handle.net/10803/666882
dc.description.abstract
Self-assembled monolayers (SAMs) on gold surfaces have been designed, processed, characterized and used for specific biological studies. The studies performed include the control of lipid bilayer diffusion, cell adhesion and vascularization studies and also the creation of antimicrobial surfaces. More specifically, dynamic SAMs on surfaces whose properties can be modified with an electrochemical external stimulus have been developed and used to interrogate biological systems. The developed platform has been applied to two different applications to overcome present challenges when performing biological studies. Firstly, in Chapter 2, the design and synthesis of all the molecules needed to develop an electroactive platform, its processing as SAMs and the optimization of the surface confined redox process between a non-reactive Hydroquinone (HQ) termination and its corresponding reactive Benzoquinone (BQ) is reported. Two different interfacial reactions taking place on the electroactivated surfaces were studied in detail; the Diels-Alder (DA) and the Michael Addition (MA) interfacial reactions, with cyclopentadiene (Cp) or thiol tagged molecules, respectively. The comparative study between DA and MA as surface functionalization strategies with a temporal control reveal that even though MA is not commonly used for this purpose it offers an attractive strategy for stimulus activated functionalization for biological applications. In Chapter 3, the developed platform has been used to achieve a temporal control of cell adhesion and in this way mimic in vivo conditions more accurately. Cell adhesion plays fundamental roles in biological functions and as such, it is important to control cell adhesion on materials used for biomedical applications. Towards this aim, the dynamic interface developed has been used to immobilize cell adhesion promoting peptides through the two different interfacial reactions, namely the DA and the MA reaction, and a comparative study has been carried out. Moreover, a study involving immobilized VEGF-mimicking peptide Qk has been conducted demonstrating the possibility of using the novel peptide for directing cell differentiation into tubular networks for in vitro platforms, by attaching them on a surface. In Chapter 4, we have used the developed electroactive interface to control the dynamics of lipid bilayers as cell membrane models, designed for transmembrane protein characterization in a more in vivo like environment. Specifically, electroactive SAMs have been used to control the moment in which tethering of lipid bilayer deposited on them occurs and consequently decrease its diffusion. In this way, proteins and lipids can maintain their fluidity until tethering is desired, a useful platform for transmembrane protein characterization. iii Finally, in Chapter 5, a surface biofunctionalization strategy also based on SAMs has been used to produce a bactericidal surface by successfully immobilizing novel antimicrobial proteins produced by recombinant DNA technology. This is relevant in view of the verge of an imminent antibiotics crisis. To confirm the antimicrobial activity and biofilm growth prevention of these surfaces, a biofilm assay was performed demonstrating that proteins retain their antimicrobial effect when immobilized. All these strategies open new possibilities for controlled biomolecule immobilization for fundamental biological studies and for applications in biotechnology, in the interface of materials science and biology.
en_US
dc.format.extent
217 p.
en_US
dc.format.mimetype
application/pdf
dc.language.iso
eng
en_US
dc.publisher
Universitat Autònoma de Barcelona
dc.rights.license
L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
*
dc.source
TDX (Tesis Doctorals en Xarxa)
dc.subject
Monocapes auto-asssemblades
en_US
dc.subject
Monocapaas asutoensambladas
en_US
dc.subject
Self-assembled monolayers
en_US
dc.subject
Monocapes electroactive
en_US
dc.subject
Monocapas electroactivas
en_US
dc.subject
Electroactive SAMs
en_US
dc.subject
Interfícies dinàmiques
en_US
dc.subject
Intercaras dinámicas
en_US
dc.subject
Dynamic interfaces
en_US
dc.subject.other
Ciències Experimentals
en_US
dc.title
Self-assembled monolayers for biological applications: design, processing, characterization and biological studies
en_US
dc.type
info:eu-repo/semantics/doctoralThesis
dc.type
info:eu-repo/semantics/publishedVersion
dc.subject.udc
54
en_US
dc.contributor.authoremail
adrikyvik@gmail.coom
en_US
dc.contributor.director
Ratera Bastardas, Imma
dc.contributor.tutor
Palet i Ballús, Cristina
dc.embargo.terms
24 mesos
en_US
dc.rights.accessLevel
info:eu-repo/semantics/openAccess


Documents

ark1de1.pdf

11.19Mb PDF

This item appears in the following Collection(s)