Implicaciones del calcio extracelular y su receptor en mebrana (CaSR) en la angiogénesis y la osteogénesis. Relevancia en ingeniería tisular.

dc.contributor
Universitat Politècnica de Catalunya. Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial
dc.contributor.author
González Vázquez, Arlyng Gyveth
dc.date.accessioned
2014-02-06T10:18:01Z
dc.date.available
2014-02-06T10:18:01Z
dc.date.issued
2013-09-26
dc.identifier.uri
http://hdl.handle.net/10803/129728
dc.description.abstract
Bone injury is a major health problem nowadays. Bone tissue engineering is a promising strategy to solve it. In this field many reports pointed out the role of scaffolds containing a calcium phosphate glass, such as G5, in the promotion of bone regeneration. These kinds of biomaterials release Ca2+ during degradation. Moreover, extracellular calcium (Ca2+0) is able to activate a special extracellular receptor called Calcium Sensing Receptor (CaSR), which can modulate migration, chemotaxis and differentiation. In that sense, present work aims to evaluate the role of extracellular calcium and CaSR as key factor in the induction of bone regeneration. We isolated endothelial progenitor cells (EPCs) and Mesenchymal Stromal cells (MSCs) from young rat’s bone marrow and they were stimulated with 10 mM Ca2+0. Results provided strong evidences about the role of Ca2+0 and CaSR in the modulation of chemotaxis, and angiogenic differentiation on EPCs, whilst on MSCs calcium exerted osteoinduction and proliferation through the CaSR. Furthermore, using biodegradable releasing calcium composite (PLA/G5) was measured the ability of that kind of biomaterials in the induction of bone and blood vessels formation in vivo. Finally, using a shell-less chick embryo model was compared the effect of the Ca2+0 and the ionic compound released by PLA/G5, demonstrating that the released Ca2+0 is the key factor in the promotion of angiogenesis and bone formation in vivo. The knowledge developed during this project will be a valuable tool to enhance the efficiency of biomaterials used in bone tissue engineering
eng
dc.format.extent
204 p.
dc.format.mimetype
application/pdf
dc.language.iso
spa
dc.publisher
Universitat Politècnica de Catalunya
dc.rights.license
L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
*
dc.source
TDX (Tesis Doctorals en Xarxa)
dc.title
Implicaciones del calcio extracelular y su receptor en mebrana (CaSR) en la angiogénesis y la osteogénesis. Relevancia en ingeniería tisular.
dc.type
info:eu-repo/semantics/doctoralThesis
dc.type
info:eu-repo/semantics/publishedVersion
dc.subject.udc
576
cat
dc.subject.udc
616.7
cat
dc.subject.udc
620
cat
dc.contributor.director
Engel López, Elisabeth
dc.embargo.terms
cap
dc.rights.accessLevel
info:eu-repo/semantics/openAccess
dc.identifier.dl
B 5034-2014


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