Cleanroom-Free Nanoelectrodes for Ultra-Sensitive Detection: From DNA Sensing to Electrochemical Lateral Flow Assay

Author

Abarintos Rentino, Vernalyn

Director

Piper , Andrew

Merkoçi, Arben

Tutor

Gòdia Casablancas, Francesc

Date of defense

2025-03-03

Pages

242 p.



Doctorate programs

Universitat Autònoma de Barcelona. Programa de Doctorat en Biotecnologia

Abstract

The primary aim of my PhD thesis involves designing, developing, and implementing cleanroom-free nanoelectrodes. One of the main objectives is to devise a fabrication technique for producing nanoelectrodes that is both inexpensive and readily available. The study emphasizes using materials and equipment commonly found in research laboratories, making the technology more widely available and reducing the financial barrier for researchers worldwide. Another objective is to demonstrate how these nanoelectrodes can be combined into more complex devices, such as complete three-electrode systems. By integrating these nanoelectrodes into an electrochemical lateral flow assay (eLFA) system, this work seeks to enhance the sensitivity and reliability of biomarker detection for diagnostic purposes.


The primary aim of my PhD thesis involves designing, developing, and implementing cleanroom-free nanoelectrodes. One of the main objectives is to devise a fabrication technique for producing nanoelectrodes that is both inexpensive and readily available. The study emphasizes using materials and equipment commonly found in research laboratories, making the technology more widely available and reducing the financial barrier for researchers worldwide. Another objective is to demonstrate how these nanoelectrodes can be combined into more complex devices, such as complete three-electrode systems. By integrating these nanoelectrodes into an electrochemical lateral flow assay (eLFA) system, this work seeks to enhance the sensitivity and reliability of biomarker detection for diagnostic purposes.


The primary aim of my PhD thesis involves designing, developing, and implementing cleanroom-free nanoelectrodes. One of the main objectives is to devise a fabrication technique for producing nanoelectrodes that is both inexpensive and readily available. The study emphasizes using materials and equipment commonly found in research laboratories, making the technology more widely available and reducing the financial barrier for researchers worldwide. Another objective is to demonstrate how these nanoelectrodes can be combined into more complex devices, such as complete three-electrode systems. By integrating these nanoelectrodes into an electrochemical lateral flow assay (eLFA) system, this work seeks to enhance the sensitivity and reliability of biomarker detection for diagnostic purposes.

Keywords

LFA; POC; Nanoelectrode

Subjects

09 - Manuscripts. Rare and remarkable works

Knowledge Area

Tecnologies

Documents

This document contains embargoed files until 2027-03-03

Rights

L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-sa/4.0/
L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-sa/4.0/

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